Use of adenovirus for ectopic gene expression inXenopus
نویسندگان
چکیده
منابع مشابه
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INTRODUCTION ........................................ 419 GENE ORGANIZATION ........................................ 419 TEMPORAL EXPRESSION OF EARLY GENES ........................................ 420 TRANSCRIPTION CONTROL........................................ 421 ACTIVATION OF TRANSCRIPTION BY ElA 421 Mechanism for E1A Action ........................................ 423 E2F Factor .............
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Objective(s) Recombinant adenoviruses are currently used for a variety of purposes, including in vitro gene transfer, in vivo vaccination, and gene therapy. Ability to infect many cell types, high efficiency in gene transfer, entering both dividing and non dividing cells, and growing to high titers make this virus a good choice for using in various experiments. In the present experiment, a reco...
متن کاملEfficient ectopic gene expression targeting chick mesoderm.
The chick model has been instrumental in illuminating genes that regulate early vertebrate development and pattern formation. Targeted ectopic gene expression is critical to dissect further the complicated gene interactions that are involved. In an effort to develop a consistent method to ectopically introduce and focally express genes in chick mesoderm, we evaluated and optimized several gene ...
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ژورنال
عنوان ژورنال: Developmental Dynamics
سال: 2009
ISSN: 1058-8388,1097-0177
DOI: 10.1002/dvdy.21932